Article
Identification of CD36 molecular features required for its in vitro angiostatic activity.
FASEB journal : official publication of the Federation of American Societies for Experimental Biology - 1 Oct 2005
Primo Luca, Ferrandi Chiara, Roca Cristina, Marchiò Serena, di Blasio Laura, Alessio Massimo, Bussolino Federico
Abstract excerpt
Thrombospondin-1 (TSP-1), a natural inhibitor of angiogenesis, acts directly on endothelial cells (EC) via CD36 to inhibit their migration and morphogenesis induced by basic fibroblast growth factor. Here we show that CD36 triggered by TSP-1 inhibits in vitro angiogenesis stimulated by vascular endothelial growth factor-A (VEGF-A). To demonstrate that the TSP-1 inhibitory signal was mediated by CD36, we...
Topics
- Amino Acid Sequence
- Angiostatic Proteins
- Apoptosis
- CD36 Antigens
- Cell Movement
- Cell Separation
- Cells, Cultured
- Cytoplasm
- DNA, Complementary
- Down-Regulation
- Flow Cytometry
- Humans
- Immunoblotting