Article
High functional overlap between MluI cell-cycle box binding factor and Swi4/6 cell-cycle box binding factor in the G1/S transcriptional program in Saccharomyces cerevisiae.
Genetics - 1 Sept 2005
Bean James M, Siggia Eric D, Cross Frederick R
Abstract excerpt
In budding yeast, many genes are induced early in the cell cycle. Induction of these genes has been predominantly attributed to two transcription factors, Swi4-Swi6 (SBF) and Mbp1-Swi6 (MBF). Swi4 and Mbp1 are related DNA-binding proteins with dissimilar target sequences. For most G1/S-regulated genes that we tested in a cdc20 block-release protocol for cell-cycle synchronization, removal of both Swi4 and Mbp1...
Topics
- Binding Sites
- Blotting, Northern
- Cell Cycle
- DNA-Binding Proteins
- G1 Phase
- Gene Deletion
- Gene Expression Regulation, Fungal
- Mutation
- Promoter Regions, Genetic
- Protein Binding
- RNA, Messenger
- S Phase
- Saccharomyces cerevisiae