Rapid identification and strain-typing of respiratory pathogens for epidemic surveillance.

doi:10.1073/pnas.0409920102

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Article

Rapid identification and strain-typing of respiratory pathogens for epidemic surveillance.

Proceedings of the National Academy of Sciences of the United States of America - 31 May 2005

Ecker David J, Sampath Rangarajan, Blyn Lawrence B, Eshoo Mark W, Ivy Cristina, Ecker Joseph A, Libby Brian, Samant Vivek, Sannes-Lowery Kristin A, Melton Rachael E, Russell Kevin, Freed Nikki, Barrozo Chris, Wu Jianguo, Rudnick Karl, Desai Anjali, Moradi Emily, Knize Duane J, Robbins David W, Hannis James C, Harrell Patina M, Massire Christian, Hall Thomas A, Jiang Yun, Ranken Raymond, Drader Jared J, White Neill, McNeil John A, Crooke Stanley T, Hofstadler Steven A

Abstract excerpt

Epidemic respiratory infections are responsible for extensive morbidity and mortality within both military and civilian populations. We describe a high-throughput method to simultaneously identify and genotype species of bacteria from complex mixtures in respiratory samples. The process uses electrospray ionization mass spectrometry and base composition analysis of PCR amplification products from highly conserved...

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PMID: 15911764
DOI: 10.1073/pnas.0409920102
PMCID: PMC1138257

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