Article
Association of human follitropin (FSH) receptor with splicing variant of human lutropin/choriogonadotropin receptor negatively controls the expression of human FSH receptor.
Molecular endocrinology (Baltimore, Md.) - 1 Aug 2005
Yamashita Soichi, Nakamura Kazuto, Omori Yuki, Tsunekawa Katsuhiko, Murakami Masami, Minegishi Takashi
Abstract excerpt
A splice variant of human lutropin (LH)/choriogonadotropin (CG)-receptor [hLHR(exon 9)] that lacks exon 9 was previously cloned in the corpus luteum of a woman with a normal menstrual cycle. Supported by a detergent-soluble binding assay and a receptor biotinylation experiment, the receptor binding assay shows hLHR(exon 9) is neither expressed at the cell surface nor has the capability of binding to hCG. In...
Topics
- Alternative Splicing
- Biotinylation
- Blotting, Western
- Cell Line
- Cyclic AMP
- DNA, Complementary
- Detergents
- Electrophoresis, Polyacrylamide Gel
- Endoplasmic Reticulum
- Exons
- Female
- Gene Expression Regulation
- Genetic Vectors