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A duplex PCR assay for detection and genotyping of Herpes simplex virus in cerebrospinal fluid.

Molecular and cellular probes - 1 Aug 1999

González-Villaseñor L I

Abstract excerpt

A duplex polymerase chain reaction (PCR) assay for the detection and genotyping of Herpes simplex virus (HSV) 1 and 2 from cerebrospinal fluid (CFS) of infants was developed. The glycoprotein D (gD) gene of HSV was selected as a target for amplification. The assay is highly specific, sensitive and reproducible. Herpes simplex virus detection is performed by agarose gel electrophoresis and Southern blot using a...

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PMID: 10441204
DOI: 10.1006/mcpr.1999.0253

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PubMed metadata and Literature Corpus relationships. Corpus snapshot updated 7 Sept 2026. Manifest publication-graph-cohort-2026-09-08.1.

Source receipt: pubmed, retrieved 7 Sept 2026.

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