Article
Generation of a vector system facilitating cloning of DMBT1 variants and recombinant expression of functional full-length DMBT1.
Protein expression and purification - 1 Jun 2005
End Caroline, Lyer Stefan, Renner Marcus, Stahl Cordula, Ditzer Jutta, Holloschi Andreas, Kuhn Hella-M, Flammann Heiko T, Poustka Annemarie, Hafner Mathias, Mollenhauer Jan, Kioschis Petra
Abstract excerpt
Deleted in malignant brain tumours 1 (DMBT1) codes for a approximately 340kDa glycoprotein with highly repetitive scavenger receptor cysteine-rich (SRCR) domains. DMBT1 was implicated in cancer, defence against viral and bacterial infections, and differentiation of epithelial cells. Recombinant expression and purification of DMBT1 is an essential step for systematic standardized functional research and towards...
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