Article
Characterization of genes encoding metal tolerance proteins isolated from Nicotiana glauca and Nicotiana tabacum.
Biochemical and biophysical research communications - 3 Jun 2005
Shingu Yoshinori, Kudo Tomoko, Ohsato Shuichi, Kimura Makoto, Ono Yoshiro, Yamaguchi Isamu, Hamamoto Hiroshi
Abstract excerpt
We have isolated a metal tolerance protein (MTP) gene, NgMTP1, from Nicotiana glauca (a potential phytoremediator plant) and two MTP genes, NtMTP1a and NtMTP1b, from Nicotiana tabacum. These three genes shared approximately 95% homology at the amino acid level. Heterologous expression of any of these three genes complemented Zn and Co tolerance in yeast mutants to a similar extent. In yeast, these proteins were...
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