Article
Identification and expression analyses of two genes encoding putative low-affinity nitrate transporters from Nicotiana plumbaginifolia.
Plant molecular biology - 1 Jan 2001
Fraisier V, Dorbe M F, Daniel-Vedele F
Abstract excerpt
Higher plants have both high- and low-affinity nitrate uptake systems (HATS and LATS respectively). Here we report the isolation and characterization of two genes, NpNRT1.1 and NpNRT1.2, from Nicotiana plumbaginifolia whose structural features suggest that they both belong to the NRT1 gene family, which is involved in the LATS. Amino acid sequence alignment showed that the N. plumbaginifolia proteins have greater...
Topics
- Amino Acid Sequence
- Anion Transport Proteins
- Bacterial Proteins
- Binding, Competitive
- Blotting, Northern
- Blotting, Southern
- Carrier Proteins
- DNA, Complementary
- DNA, Plant
- Gene Expression Regulation, Plant