Article
[Effect of recombinant plasmid of Helicobacter pylori ureB gene on gastric epithelial cell].
Wei sheng wu xue bao = Acta microbiologica Sinica - 1 Feb 2005
Zhang Jing, She Fei-fei, Chen Yue-xiu, Chen Hao
Abstract excerpt
To investigate the effect of recombinant plasmid of Helicobacter pylori ureB gene on gastric epithelial cell. The full length sequence of ureB gene from NCTC11637 was amplified by PCR. The recombinant plasmid was constructed by cloning the open reading frame (ORF) of ureB into the eukaryotic expression vector pcDNA3.1, and was transfected SGC-7901 cells, then the clones resisting Hygromycin were screened. mRNA...
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