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AMPLIFICATION OF HUMAN DNA BY PRIMERS TARGETED TO LEISHMANIA KINETOPLAST DNA AND POST-GENOME CONSIDERATIONS IN THE DETECTION OF PARASITES BY A POLYMERASE CHAIN REACTION

1 Apr 2005

Abstract excerpt

We evaluated the Leishmania Viannia-specific primers B1-B2 to detect Leishmania in normal skin and peripheral blood monocytes of patients with active cutaneous leishmaniasis. Southern blotting and sequencing of polymerase chain reaction (PCR) products confirmed the specificity of kinetoplast DNA (kDNA) amplification from tissue fluid from healthy skin, whereas the PCR with monocytes also amplified a human...

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PMID: 15827280
DOI: 10.4269/ajtmh.2005.72.423

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PubMed metadata and Literature Corpus relationships. Corpus snapshot updated 7 Sept 2026. Manifest publication-graph-cohort-2026-09-08.1.

Source receipt: pubmed, retrieved 7 Sept 2026.

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