Article
Rapid phenotypic characterization of Salmonella enterica strains by pyrolysis metastable atom bombardment mass spectrometry with multivariate statistical and artificial neural network pattern recognition.
Journal of microbiological methods - 1 Jun 2005
Wilkes Jon G, Rushing Larry, Nayak Rajesh, Buzatu Dan A, Sutherland John B
Abstract excerpt
Pyrolysis mass spectrometry was investigated for rapid characterization of bacteria. Spectra of Salmonella were compared to their serovars, pulsed-field gel electrophoresis (PFGE) patterns, antibiotic resistance profiles, and MIC values. Pyrolysis mass spectra generated via metastable atom bombardment were analyzed by multivariate principal component-discriminant analysis and artificial neural networks (ANNs)....
Topics
- Bacterial Typing Techniques
- Bacteriological Techniques
- DNA, Bacterial
- Drug Resistance, Bacterial
- Electrophoresis, Gel, Pulsed-Field
- Mass Spectrometry
- Multivariate Analysis
- Neural Networks, Computer
- Pattern Recognition, Automated
- Phenotype
- Principal Component Analysis