Article
Rapid Species Identification Within theMycobacterium chelonae–abscessusGroup by High-Resolution Melting Analysis ofhsp65PCR Products
1 Jan 2005
Abstract excerpt
Polymerase chain reaction (PCR) amplification of the heat shock protein 65 (hsp65) gene followed by high-resolution melting analysis with LCGreen I (Idaho Technology, Salt Lake City, UT) was used to differentiate the mycobacteria species Mycobacterium chelonae, Mycobacterium abscessus, and Mycobacterium immunogenum in less than 20 minutes. A 105-base-pair amplicon that clustered the different species by predicted...
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