Article
New pOp/LhG4 vectors for stringent glucocorticoid-dependent transgene expression in Arabidopsis.
The Plant journal : for cell and molecular biology - 1 Mar 2005
Craft Judith, Samalova Marketa, Baroux Celia, Townley Helen, Martinez Alberto, Jepson Ian, Tsiantis Miltos, Moore Ian
Abstract excerpt
To facilitate glucocorticoid-inducible transgene expression from the pOp promoter in Arabidopsis the ligand-binding domain of a rat glucocorticoid receptor (GR LBD) was fused to the amino terminus of the synthetic transcription factor LhG4 to generate LhGR-N. Fusions bearing the GR LBD at other positions in LhG4 exhibited incomplete repression or inefficient induction. LhGR-N was stringently repressed in the...
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