Article
Mutational study of sapovirus expression in insect cells.
Virology journal - 23 Feb 2005
Hansman Grant S, Katayama Kazuhiko, Oka Tomoichiro, Natori Katsuro, Takeda Naokazu
Abstract excerpt
Human sapovirus (SaV), an agent of human gastroenteritis, cannot be grown in cell culture, but expression of the recombinant capsid protein (rVP1) in a baculovirus expression system results in the formation of virus-like particles (VLPs). In this study we compared the time-course expression of two different SaV rVP1 constructs. One construct had the native sequence (Wt construct), whereas the other had two...
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