Article
Standardization of a technique for analysing the frequency of parasite-specific cytotoxic T lymphocyte precursors in cattle immunized with Theileria parva.
Parasite immunology - 1 Mar 1992
Taracha E L, Goddeeris B M, Scott J R, Morrison W I
Abstract excerpt
A limiting dilution microculture system was optimized to quantify the frequency of Theileria parva-specific cytotoxic T lymphocyte precursors (CTLp) in peripheral blood mononuclear cells (PBMC) from immune cattle. Optimal results were obtained with responder cell input levels ranging from 2 x 10(4)/well to 6.25 x 10(2)/well, along with 1-5 x 10(3)/well stimulator cells in standard supplemented RPMI 1640 medium...
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