Article
Standardization of a technique for analysing the frequency of parasite-specific cytotoxic T lymphocyte precursors in cattle immunized with Theileria parva.
Parasite immunology - 1 Mar 1992
Taracha E L, Goddeeris B M, Scott J R, Morrison W I
Abstract excerpt
A limiting dilution microculture system was optimized to quantify the frequency of Theileria parva-specific cytotoxic T lymphocyte precursors (CTLp) in peripheral blood mononuclear cells (PBMC) from immune cattle. Optimal results were obtained with responder cell input levels ranging from 2 x 10(...
Topics
- Animals
- Cattle
- Cytotoxicity Tests, Immunologic
- Female
- Immunization
- Kinetics
- Leukocytes, Mononuclear
- Male
- Phenotype
- Reproducibility of Results
- Sensitivity and Specificity
- T-Lymphocytes, Cytotoxic
- Theileria parva
- Theileriasis