Article
Utility of environmental primers targeting ancient enzymes: methylotroph detection in Lake Washington.
Microbial ecology - 1 Nov 2004
Kalyuzhnaya M G, Lidstrom M E, Chistoserdova L
Abstract excerpt
Methods have been explored for detection of methylotrophs in natural samples, using environmental primers based on genes involved in the tetrahydromethanopterin (H4MPT)-linked C1 transfer pathway. The underlying hypotheses were that the H4MPT-linked pathway is an ancient methylotrophy pathway, based on gene divergence, and that primers targeting more divergent genes will detect a broader variety of methylotrophs...
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