Article
Design and evaluation of novel primers for the detection of genes encoding diverse enzymes of methylotrophy and autotrophy.
Polish journal of microbiology - 1 Jan 2012
Hung Wei-Lian, Wade William G, Chen Yin, Kelly Donovan P, Wood Ann P
Abstract excerpt
The phylogenetic significance of the diversity of key enzymes of methylotrophic and autotrophic metabolism is discussed. Primers for these key enzymes were designed using gene sequences encoding methanol dehydrogenase (mxaF; using subsets from database sequences for 22 Bacteria), hydroxypyruvate reductase (hpr; 36 sequences), methylamine dehydrogenase (mauA; 12 sequences), methanesulfonate monooxygenase (msmA;...
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