Article
[Detection of a mutant HSV-1 in a patient presenting efflorescence on an eye lid].
Klinicka mikrobiologie a infekcni lekarstvi - 1 Dec 2004
Suchánková Alena, Zezulák Jirí
Abstract excerpt
A 9-month-old boy with impetigious blepharitis of the right eye was admitted to the Department of children and adolescents of the Kralovske Vinohrady University Hospital. NA isolated from lesion fluid was screened using real-time PCR based HSV 1/2 detection kit (Roche). Differentiation between HSV types 1 and 2 in this assay is based on variation of double-stranded DNA melting temperature (Tm), being 54 degrees C...
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