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Article

Failure to genotype herpes simplex virus by real-time PCR assay and melting curve analysis due to sequence variation within probe binding sites.

Journal of clinical microbiology - 1 May 2003

Anderson Trevor P, Werno Anja M, Beynon Kirsten A, Murdoch David R

Abstract excerpt

Real-time PCR with melting curve analysis of PCR products is a rapid procedure for detecting and genotyping herpes simplex virus (HSV). When testing mucocutaneous samples for HSV by a real-time PCR assay targeting the DNA polymerase gene, we found that some PCR products had atypical melting curves that did not conform to the expected melting temperatures for HSV type 1 or 2. Sequence analysis showed that these...

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Identifiers

PMID: 12734260
DOI: 10.1128/JCM.41.5.2135-2137.2003
PMCID: PMC154719

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PubMed metadata and Literature Corpus relationships. Corpus snapshot updated 7 Sept 2026. Manifest publication-graph-cohort-2026-09-07.1.

Source receipt: pubmed, retrieved 7 Sept 2026.

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