Article
Complementation of sugary-1 phenotype in rice endosperm with the wheat isoamylase1 gene supports a direct role for isoamylase1 in amylopectin biosynthesis.
Plant physiology - 1 Jan 2005
Kubo Akiko, Rahman Sadequr, Utsumi Yoshinori, Li Zhongyi, Mukai Yasuhiko, Yamamoto Maki, Ugaki Masashi, Harada Kyuya, Satoh Hikaru, Konik-Rose Christine, Morell Matthew, Nakamura Yasunori
Abstract excerpt
To examine the role of isoamylase1 (ISA1) in amylopectin biosynthesis in plants, a genomic DNA fragment from Aegilops tauschii was introduced into the ISA1-deficient rice (Oryza sativa) sugary-1 mutant line EM914, in which endosperm starch is completely replaced by phytoglycogen. A. tauschii is the D genome donor of wheat (Triticum aestivum), and the introduced fragment effectively included the gene for ISA1 for...
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