Article
Cold-active DnaK of an Antarctic psychrotroph Shewanella sp. Ac10 supporting the growth of dnaK-null mutant of Escherichia coli at cold temperatures.
Extremophiles : life under extreme conditions - 1 Apr 2005
Yoshimune Kazuaki, Galkin Andrey, Kulakova Ljudmila, Yoshimura Tohru, Esaki Nobuyoshi
Abstract excerpt
Shewanella sp. Ac10 is a psychrotrophic bacterium isolated from the Antarctica that actively grows at such low temperatures as 0 degrees C. Immunoblot analyses showed that a heat-shock protein DnaK is inducibly formed by the bacterium at 24 degrees C, which is much lower than the temperatures causing heat shock in mesophiles such as Escherichia coli. We found that the Shewanella DnaK (SheDnaK) shows much higher...
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