Discover Community Create You

Loading publication...

Noodle always counts identifier-free feature events for service operation. With your permission, Google Analytics can also estimate audience and engagement. Search text, publication identifiers, cookies, IP addresses and browser fingerprints are never included in Noodle's first-party event counters.

Privacy details

Article

Use of REMI and Agrobacterium-mediated transformation to identify pathogenicity mutants of the biocontrol fungus, Coniothyrium minitans.

FEMS microbiology letters - 15 Dec 2004

Rogers Christopher W, Challen Michael P, Green Jonathan R, Whipps John M

Abstract excerpt

Restriction enzyme mediated integration (REMI) and Agrobacterium-mediated transformation (ATMT) were used to transform protoplasts or germinated conidia of the mycoparasite Coniothyrium minitans to hygromycin resistance. Using REMI, up to 32 transformants mug DNA(-1) were obtained, while 37.8 transformants 5 x 10(5) germlings(-1) were obtained using ATMT. Single-copy integrations occurred in 8% and 40% of REMI...

Read the complete abstract on PubMed

Topics

Share this publication in a Topic to start or enrich a Post.

Share to Agrobacterium tumefaciens
Share to Ascomycota

Methodology Data sources Editorial policy Privacy About Contact Helena Bioinformatics

Share to Mutation

Share to Pest Control, Biological

Share to Plant Diseases

Share to Restriction Mapping

Share to Transformation, Bacterial

Share to Virulence

Source

Identifiers

PMID: 15598534
DOI: 10.1016/j.femsle.2004.10.022

Provenance

PubMed metadata and Literature Corpus relationships. Corpus snapshot updated 7 Sept 2026. Manifest publication-graph-cohort-2026-09-07.1.

Source receipt: pubmed, retrieved 7 Sept 2026.

Related research

Semantic proximity does not establish scientific evidence.

Research trail→

Click a neighbor to travelStep 1 · 9 closest

Use of REMI and Agrobacterium-mediated transformation to identify pathogenicity mutants of the biocontrol fungus, Coniothyrium minitans.PMID 15598534Read ↗

Transformation of Coniothyrium minitans, a parasite of Sclerotinia sclerotiorum, with Agrobacterium tumefaciens.Semantic proximity Read ↗

Use of Coniothyrium minitans as a biocontrol agent and some molecular aspects of sclerotial mycoparasitismSemantic proximity Read ↗

Disruption of the Coniothyrium minitans PIF1 DNA helicase gene impairs growth and capacity for sclerotial mycoparasitism.Semantic proximity Read ↗

Phosphoribosylamidotransferase, the first enzyme for purine de novo synthesis, is required for conidiation in the sclerotial mycoparasite Coniothyrium minitans.Semantic proximity Read ↗

Molecular cloning, characterization and expression analysis of a pacC homolog in the mycoparasite Coniothyrium minitansSemantic proximity Read ↗

REMI-induced mutants of Mycosphaerella zeae-maydis lacking the polyketide PM-toxin are deficient in pathogenesis to corn.Semantic proximity Read ↗

Disruption of heat shock factor 1 reduces the formation of conidia and thermotolerance in the mycoparasitic fungus Coniothyrium minitans.Semantic proximity Read ↗

Breeding of mycoparasitic Trichoderma strains for heavy metal resistance.Semantic proximity Read ↗

Degradation of cyanide by Trichoderma mutants constructed by restriction enzyme mediated integration (REMI).Semantic proximity Read ↗

⌁Select a neighboring publication to make it the new centre.