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Rapid identification and differentiation of fungal DNA in dermatological specimens by LightCycler PCR

19 Nov 2004

Abstract excerpt

The aim was to develop a LightCycler PCR method for the rapid detection and differentiation of fungal DNA in dermatological specimens such as skin scales and skin swabs. LightCycler PCR assays were established for seven primer sets specific for fungal DNA. For each primer set LightCycler melting points were defined by amplification of DNA from 21 fungi and sensitivity was determined by amplification of serial...

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PMID: 15585499
DOI: 10.1099/jmm.0.45779-0

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