Article
Spontaneous mutation conferring the ability to catabolize mannopine in Agrobacterium tumefaciens.
Journal of bacteriology - 1 Apr 1992
LaPointe G, Nautiyal C S, Chilton W S, Farrand S K, Dion P
Abstract excerpt
Two nopaline-type strains of Agrobacterium tumefaciens, C58 and T37, as well as strain A136, which is a Ti plasmid-cured derivative of strain C58, gave rise to spontaneous mutants that were able to grow on mannopine. The observation of mutagenesis with strain A136 demonstrated that the ability to acquire this new catabolic potential was independent of the presence of a Ti plasmid. The mutants were isolated after...
Topics
- Agrobacterium tumefaciens
- Blotting, Southern
- Cloning, Molecular
- DNA, Bacterial
- Genes, Bacterial
- Mannitol
- Mutation
