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Identification of <i>Aspergillus fumigatus</i> and Related Species by Nested PCR Targeting Ribosomal DNA Internal Transcribed Spacer Regions

1 Jun 2001

Abstract excerpt

Aspergillus fumigatus is the most common species that causes invasive aspergillosis. In order to identify A. fumigatus, partial ribosomal DNA (rDNA) from two to six strains of five different Aspergillus species was sequenced. By comparing sequence data from GenBank, we designed specific primer pairs targeting rDNA internal transcribed spacer (ITS) regions of A. fumigatus. A nested PCR method for identification of...

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PMID: 11376067
DOI: 10.1128/jcm.39.6.2261-2266.2001

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