Article
Mapping Ds insertions in barley using a sequence-based approach.
Molecular genetics and genomics : MGG - 1 Sept 2004
Cooper L D, Marquez-Cedillo L, Singh J, Sturbaum A K, Zhang S, Edwards V, Johnson K, Kleinhofs A, Rangel S, Carollo V, Bregitzer P, Lemaux P G, Hayes P M
Abstract excerpt
A transposon tagging system, based upon maize Ac/Ds elements, was developed in barley (Hordeum vulgaresubsp. vulgare). The long-term objective of this project is to identify a set of lines with Ds insertions dispersed throughout the genome as a comprehensive tool for gene discovery and reverse genetics. AcTPase and Ds-bar elements were introduced into immature embryos of Golden Promise by biolistic...
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