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Article

Variable Surface Glycoprotein RoTat 1.2 PCR as a specific diagnostic tool for the detection of Trypanosoma evansiinfections

17 Sept 2004

Abstract excerpt

BACKGROUND: Based on the recently sequenced gene coding for the Trypanosoma evansi (T. evansi) RoTat 1.2 Variable Surface Glycoprotein (VSG), a primer pair was designed targeting the DNA region lacking homology to other known VSG genes. A total of 39 different trypanosome stocks were tested using the RoTat 1.2 based Polymerase Chain Reaction (PCR). RESULTS: This PCR yielded a 205 bp product in all T. evansi and...

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PMID: 15377385
DOI: 10.1186/1475-9292-3-3

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