Article
Agrobacterium-mediated genetic transformation of Perilla frutescens.
Plant cell reports - 1 Nov 2004
Kim Kyung-Hwan, Lee Yeon-Hee, Kim Donghern, Park Yong-Hwan, Lee Jai-Youl, Hwang Young-Soo, Kim Yong-Hwan
Abstract excerpt
A reproducible plant regeneration and an Agrobacterium tumefaciens-mediated genetic transformation protocol were developed for Perilla frutescens (perilla). The largest number of adventitious shoots were induced directly without an intervening callus phase from hypocotyl explants on MS medium supplemented with 3.0 mg/l 6-benzylaminopurine (BA). The effects of preculture and extent of cocultivation were examined...
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