Article
Detection of hepatitis C virus by a user-developed reverse transcriptase-PCR and use of amplification products for subsequent genotyping.
Journal of clinical virology : the official publication of the Pan American Society for Clinical Virology - 1 Oct 2004
Tang Yi-Wei, Li Haijing, Roberto Ann, Warner Diane, Yen-Lieberman Belinda
Abstract excerpt
BACKGROUND: Detection, quantitation and genotyping of hepatitis C virus (HCV) are important in selecting appropriate therapy. Current commercially available HCV genotyping kits, including sequencing-based TRUGENE HCV 5'NC and hybridization-based INNO-LiPA HCV II assays, rely on amplification products (amplicons) generated by HCV reverse-transcriptase (RT)-PCR methods such as the Roche AMPLICOR HCV test. METHODS:...
Topics
- Base Sequence
- DNA, Viral
- Genotype
- Hepacivirus
- Hepatitis C
- Humans
- Nucleic Acid Amplification Techniques
- Reverse Transcriptase Polymerase Chain Reaction
- Virology