Article
[Development of hygromycin-resistant packaging cell line for hepatitis B virus-derived vectors].
Zhonghua shi yan he lin chuang bing du xue za zhi = Zhonghua shiyan he linchuang bingduxue zazhi = Chinese journal of experimental and clinical virology - 1 Mar 2004
Hu Da-rong, Sun Dian-xing, Xiong Jin-hua, Wu Guang-hui, Hu Xue-ling, Li Juan, Fan Gong-ren, Han Ju-qiang
Abstract excerpt
OBJECTIVE: To cooperate with the study of HBV vector, hygromycin-resistant packaging cell line was developed that allows encapsidation of plasmids into HBV particles. METHODS: Free of packaging signal, HBV genome was inserted into plasmid pMEP4, which expresses the HBV structural proteins including core, pol and preS/S proteins. HepG2 cell lines were employed to transfect with the construct. Hygromycin selection...
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