Article
A study on the effects of degradation and template concentration on the amplification efficiency of the STR Miniplex primer sets.
Journal of forensic sciences - 1 Jul 2004
Chung Denise T, Drábek Jirí, Opel Kerry L, Butler John M, McCord Bruce R
Abstract excerpt
In forensic DNA analysis, the samples recovered from the crime scene are often highly degraded leading to poor PCR amplification of the larger sized STR loci. To avoid this problem, we have developed STR markers with redesigned primer sequences called "Miniplexes" to produce smaller amplicons. To assess the effectiveness of these kits, we have tested these primer sets with enzymatically degraded DNA and compared...
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