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Article

Molecular Serotyping of<i>Klebsiella</i>Species Isolates by Restriction of the Amplified Capsular Antigen Gene Cluster

1 Aug 2004

Abstract excerpt

The objective of the present work was to develop a molecular method that would enable determination of the capsular serotypes of Klebsiella isolates without the use of antiserum. PCR amplification of the capsular antigen gene cluster (cps) was followed by digestion with the restriction enzyme HincII (cps PCR-restriction fragment length polymorphism [RFLP] analysis). The profiles (C patterns) obtained for 224...

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PMID: 15297473
DOI: 10.1128/jcm.42.8.3388-3398.2004

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