Article
Rapid DNA fingerprinting to control for specimen errors in HIV testing by the polymerase chain reaction.
Molecular and cellular probes - 1 Aug 1992
Cassol S, Rudnik J, Salas T, Montpetit M, Pon R T, Sy C T, Read S, Major C, O'Shaughnessy M V
Abstract excerpt
Variable-number-tandem-repeats (VNTRs) are highly polymorphic and provide informative genetic markers for distinguishing between individuals. We have used PCR amplification of VNTR locus pMCT118 to identify mislabelled specimens submitted for HIV PCR testing. The method is rapid, can be applied to large numbers of samples and eliminates the need for radioactive probes. DNA samples (10 ng) are amplified for 25...
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