Amplification of reproducible allele markers for amplified fragment length polymorphism analysis.

Article

Amplification of reproducible allele markers for amplified fragment length polymorphism analysis.

BioTechniques - 1 Jan 1992

Sajantila A, Puomilahti S, Johnsson V, Ehnholm C

Abstract excerpt

A procedure for amplification by PCR of reproducible allele markers for amplified fragment length polymorphism (Amp-FLP) analysis is presented. We have prepared markers for the allelic products of the VNTR loci D1S80 (MCT118) and D17S30 (YNZ22) and for the hypervariable VNTR locus close to the 3'...

Topics

Alleles
Apolipoproteins B
Base Sequence
DNA
Forensic Medicine
Genetic Markers
Humans
Male
Molecular Sequence Data
Paternity
Polymerase Chain Reaction
Polymorphism, Restriction Fragment Length
Repetitive Sequences, Nucleic Acid

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