Article
Amplification of reproducible allele markers for amplified fragment length polymorphism analysis.
BioTechniques - 1 Jan 1992
Sajantila A, Puomilahti S, Johnsson V, Ehnholm C
Abstract excerpt
A procedure for amplification by PCR of reproducible allele markers for amplified fragment length polymorphism (Amp-FLP) analysis is presented. We have prepared markers for the allelic products of the VNTR loci D1S80 (MCT118) and D17S30 (YNZ22) and for the hypervariable VNTR locus close to the 3' end of the apolipoprotein B gene (apoB) by re-amplifying a mixture of PCR products from individuals with known...
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