Article
Analysis and genotyping of PCR products of the Amplicor HIV-1 kit.
Journal of virological methods - 1 Mar 1995
Barlow K L, Tosswill J H, Clewley J P
Abstract excerpt
The Roche Amplicor PCR kit was used to detect HIV-1 DNA in UK patients of known serostatus. Four false-negative and/or equivocal results were obtained from patients who were known to be anti HIV seropositive (Tosswill et al., 1994). Cells from the blood of these patients were shown to contain HIV DNA after extraction, concentration and amplification by nested PCR using primers flanking those in the kit. To...
Topics
- Base Sequence
- DNA Primers
- DNA, Viral
- Electrophoresis, Agar Gel
- False Negative Reactions
- Genes, gag
- Genotype
- HIV Seropositivity
- HIV-1
- Humans
- Molecular Sequence Data
- Oligonucleotide Probes