Article
Hydrogen photoproduction is attenuated by disruption of an isoamylase gene in Chlamydomonas reinhardtii.
The Plant cell - 1 Aug 2004
Posewitz Matthew C, Smolinski Sharon L, Kanakagiri Saradadevi, Melis Anastasios, Seibert Michael, Ghirardi Maria L
Abstract excerpt
DNA insertional transformants of Chlamydomonas reinhardtii were screened chemochromically for attenuated H(2) production. One mutant, displaying low H(2) gas photoproduction, has a nonfunctional copy of a gene that shows high homology to the family of isoamylase genes found in several photosynthetic organisms. DNA gel blotting and gene complementation were used to link this isoamylase gene to previously...
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