Article
Contribution of a low-barrier hydrogen bond to catalysis is not significant in ketosteroid isomerase.
Molecules and cells - 1 May 2015
Jang Do Soo, Choi Gildon, Cha Hyung Jin, Shin Sejeong, Hong Bee Hak, Lee Hyeong Ju, Lee Hee Cheon, Choi Kwan Yong
Abstract excerpt
Low-barrier hydrogen bonds (LBHBs) have been proposed to have important influences on the enormous reaction rate increases achieved by many enzymes. Δ(5)-3-ketosteroid isomerase (KSI) catalyzes the allylic isomerization of Δ(5)-3-ketosteroid to its conjugated Δ(4)-isomers at a rate that approaches the diffusion limit. Tyr14, a catalytic residue of KSI, has been hypothesized to form an LBHB with the oxyanion of a...
Topics
- Bacterial Proteins
- Biocatalysis
- Catalytic Domain
- Crystallography, X-Ray
- Equilenin
- Hydrogen Bonding
- Models, Molecular
- Mutation
- Protein Binding
- Proton Magnetic Resonance Spectroscopy
- Pseudomonas putida
- Steroid Isomerases
- Substrate Specificity