Article
Functional analysis of the tandem-duplicated P450 genes SPS/BUS/CYP79F1 and CYP79F2 in glucosinolate biosynthesis and plant development by Ds transposition-generated double mutants.
Plant physiology - 1 Jun 2004
Tantikanjana Titima, Mikkelsen Michael Dalgaard, Hussain Mumtaz, Halkier Barbara Ann, Sundaresan Venkatesan
Abstract excerpt
A significant fraction (approximately 17%) of Arabidopsis genes are members of tandemly repeated families and pose a particular challenge for functional studies. We have used the Ac-Ds transposition system to generate single- and double-knockout mutants of two tandemly duplicated cytochrome P450 genes, SPS/BUS/CYP79F1 and CYP79F2. We have previously described the Arabidopsis supershoot mutants in CYP79F1 that...
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