Article
Identification of a ThirdBoophilus microplus(Acari: Ixodidae) cDNA Presumptively Encoding an Acetylcholinesterase
1 May 2004
Abstract excerpt
Oligodeoxynucleotide primers, based on amino acid sequences conserved in known acetylcholinesterases (AChEs), were used in reverse-transcription polymerase chain reaction (RT-PCR) with mRNA from Boophilus microplus (Canestrini) as the template. Primer walking and rapid amplification of cDNA ends (RACE) techniques were used to complete the cDNA sequence identified by RT-PCR. The complete B. microplus cDNA sequence...
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