Article
High frequency of nucleotide misincorporations upon the processing of double-strand breaks.
DNA repair - 4 Mar 2004
Kovalchuk Igor, Pelczar Pawel, Kovalchuk Olga
Abstract excerpt
Base substitutions were detected as a consequence of double-strand break (DSB) repair in plants. The fidelity of processing free DNA ends was analyzed using a stop-codon inactivated beta-glucuronidase (uidA) reporter gene. Circular and linear plasmids carrying the inactive gene were delivered to Nicotiana plumbaginifolia protoplasts or Nicotiana tabacum leaves. Processing of breaks which occurred in close...
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