Article
A comparative evaluation of phenotypic and molecular methods for the detection of oxacillin resistance in coagulase-negative staphylococci.
Journal of infection and chemotherapy : official journal of the Japan Society of Chemotherapy - 1 Apr 2004
Ghoshal Ujjala, Prasad Kashi N, Singh Manisha, Tiwari Dinesh P, Ayyagari Archana
Abstract excerpt
Detection of oxacillin resistance in coagulase-negative staphylococci (C-NS) by phenotypic methods is often difficult. The present study compared the National Committee for Clinical Laboratory Standards (NCCLS) revised guidelines of phenotypic methods with a mecA-based polymerase chain reaction (PCR) for C-NS. Ninety clinical C-NS isolates were tested for oxacillin resistance by disk diffusion (1-microg disk),...
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