Analysis of Borderline‐Resistant Strains of Methicillin‐Resistant Staphylococcus aureus Using Polymerase Chain Reaction

doi:10.1111/j.1348-0421.1992.tb02043.x

Article

Analysis of Borderline‐Resistant Strains of Methicillin‐Resistant Staphylococcus aureus Using Polymerase Chain Reaction

1 May 1992

Abstract excerpt

Identification of methicillin-resistant Staphylococcus aureus by drug-susceptibility tests alone poses a serious problem, because a considerable number of clinical S. aureus isolates are borderline resistant to methicillin. To circumvent this problem, we have developed a quick and sensitive method of PCR amplification for the detection of mecA gene, which, coding for PBP2', is the specific genetic element of...

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