Detection of <i>Shigella</i> by a PCR Assay Targeting the <i>ipaH</i> Gene Suggests Increased Prevalence of Shigellosis in Nha Trang, Vietnam

Abstract excerpt

Shigella spp. are exquisitely fastidious gram-negative organisms which frequently escape detection by traditional culture methods. To get a more complete understanding of the disease burden caused by Shigella in Nha Trang, Vietnam, real-time PCR was used to detect Shigella DNA. Randomly selected rectal swab specimens from 60 Shigella culture-positive patients and 500 Shigella culture-negative patients detected by...

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