Article
Detection of polymorphisms of human DNA after polymerase chain reaction by miniaturized SDS-PAGE.
Forensic science international - 1 Jul 1992
Barros F, Lareu M V, Carracedo A
Abstract excerpt
PCR followed by SDS-PAGE in miniaturized non-denaturing gels permits in some cases the identification of single base pair substitutions in small DNA fragments and therefore, the study of human DNA polymorphisms. The usefulness of the system in forensic science is investigated by typing the HLA-DQA1 locus and the VNTR recognized with the probe pMCT118 (locus D1S80) and it shows to be advantageous over previously...
Topics
- Base Sequence
- Blood Stains
- DNA
- Electrophoresis, Polyacrylamide Gel
- Forensic Medicine
- HLA-DQ Antigens
- HLA-DQ alpha-Chains
- Hair
- Humans
- Molecular Sequence Data
- Nucleic Acid Amplification Techniques