Article
Factors affecting the rate of T-DNA transfer from Agrobacterium tumefaciens to Nicotiana glauca plant cells.
Plant molecular biology - 1 Sept 1992
Mozo T, Hooykaas P J
Abstract excerpt
Different factors involved in the early steps of the T-DNA transfer process were studied by using a beta-glucuronidase gene (gusA) as a reporter in Nicotiana glauca leaf disc transformation experiments. The levels of transient expression of the gusA gene in leaf discs infected with several strains or vir mutants correlated well with their virulence phenotype, except for virC mutants. The rate of T-DNA transfer...
Topics
- Agrobacterium tumefaciens
- Kinetics
- Mutation
- Plants, Toxic
- Plasmids
- Nicotiana
- Transformation, Genetic
- Virulence