Article
Mutants of Agrobacterium tumefaciens with elevated vir gene expression.
Proceedings of the National Academy of Sciences of the United States of America - 15 Aug 1991
Pazour G J, Ta C N, Das A
Abstract excerpt
Expression of Agrobacterium tumefaciens virulence (vir) genes requires virA, virG, and a plant-derived inducing compound such as acetosyringone. To identify the critical functional domains of virA and virG, a mutational approach was used. Agrobacterium A136 harboring plasmid pGP159, which contains virA, virG, and a reporter virB:lacZ gene fusion, was mutagenized with UV light or nitrosoguanidine. Survivors that...
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