Article
Intracellular transport of human lysosomal alpha-mannosidase and alpha-mannosidosis-related mutants.
The Biochemical journal - 15 Jul 2004
Hansen Gaute, Berg Thomas, Riise Stensland Hilde M F, Heikinheimo Pirkko, Klenow Helle, Evjen Gry, Nilssen Øivind, Tollersrud Ole K
Abstract excerpt
Human LAMAN (lysosomal a-mannosidase) was synthesized as a 120 kDa precursor in transfected COS cells [African-green-monkey kidney cells], which was partly secreted as a single-chain form and partly sorted to the lysosomes being subsequently cleaved into three peptides of 70, 40 and 15 kDa respectively. Both the secreted and the lysosomal forms contained endo H (endoglucosidase H)-resistant glycans, suggesting a...
Topics
- Animals
- CHO Cells
- COS Cells
- Cattle
- Cell Line
- Chlorocebus aethiops
- Cricetinae
- Genotype
- Isoenzymes
- Lysosomes
- Mannosidases
- Models, Molecular
- Mutagenesis, Site-Directed
- Phenotype