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Article

L273S missense substitution in human lysosomal acid lipase creates a new N-glycosylation site.

FEBS letters - 11 Nov 1996

Pariyarath R, Pagani F, Stuani C, Garcia R, Baralle F E

Abstract excerpt

Human lysosomal acid lipase (LAL), when expressed in HeLa cells using the Vaccinia T7 expression system, showed four major molecular forms ranging from 42 to 54 kDa. Treatment with endoglycosidase H resulted in a 42 kDa protein, indicating that the molecular weight variations were due to N-glycos...

Topics

Amino Acid Sequence
Catalysis
Glycosylation
HeLa Cells
Hexosaminidases
Humans
Lipase
Lysosomes
Molecular Weight
Mutagenesis, Site-Directed
Mutation
Recombinant Proteins
Sterol Esterase

Methodology Data sources Editorial policy Privacy About Contact Helena Bioinformatics

Transfection

Source

Identifiers

PMID: 8941718
DOI: 10.1016/s0014-5793(96)01134-9

Genes

NMS

Provenance

PubMed metadata and Literature Corpus relationships. Corpus snapshot updated 30 Aug 2026. Prepared record publication-8941718-2026-08-30.1. Manifest publication-graph-cohort-2026-08-30.1.

Source receipt: PubMed via Helena Literature Corpus, retrieved 30 Aug 2026.

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