Mechanistic insights into the kinetics of HIV-1 nucleocapsid protein-facilitated tRNA annealing to the primer binding site.

Abstract excerpt

HIV-1 reverse transcriptase uses human tRNA(Lys,3) as a primer to initiate reverse transcription. Prior to initiation, the 3' 18 nucleotides of this tRNA are annealed to a complementary sequence on the RNA genome known as the primer binding site (PBS). Here, we show that the HIV-1 nucleocapsid protein (NC) enhances this annealing by approximately five orders of magnitude in vitro, decreasing the transition state...

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