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Article

Detection of multiple beta-casein (CASB) alleles by amplification created restriction sites (ACRS).

Animal genetics - 1 Jan 1992

Lien S, Aleström P, Klungland H, Rogne S

Abstract excerpt

Direct sequencing of polymerase chain reaction (PCR) amplified DNA has been used to detect the DNA sequences for bovine beta-casein (CASB) A3 and B variants. Based on these sequences we have designed primers which create allele-specific restriction sites in the PCR product. Restriction analysis of PCR product generated in one reaction enable us to identify the A1, A2, A3 and B alleles of CASB rapidly without the...

Topics

Alleles
Animals
Base Sequence
Caseins
Cattle
Gene Amplification
Genetic Variation
Male
Molecular Sequence Data
Mutagenesis, Site-Directed
Oligonucleotides
Polymerase Chain Reaction

Methodology Data sources Editorial policy Privacy About Contact Helena Bioinformatics

Restriction Mapping

Source

Identifiers

PMID: 1503272
DOI: 10.1111/j.1365-2052.1992.tb00155.x

Provenance

PubMed metadata and Literature Corpus relationships. Corpus snapshot updated 29 Aug 2026. Prepared record publication-1503272-2026-08-29.1. Manifest publication-graph-cohort-2026-08-29.1.

Source receipt: PubMed via Helena Literature Corpus, retrieved 29 Aug 2026.

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